ELISA Flex: Human IgE (HRP)

3810-1H-6

Publications: 18

Documents

Bulk or custom format

An ELISA designed to detect the native protein – in our most adaptable kit format to match your unique setup. Combine it with your preferred substrate and buffer.

Components

Capture mAb	107
Detection mAb	182, biotin
Enzyme conjugate	Streptavidin-HRP
Standard	Native human IgE ELISA standard
Buffer/Solution	Standard reconstitution buffer

In stock

Delivery 4-9 business days

Shipping $0

Complementary products

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ELISA diluent

Product specifications

Intended use

This ELISA Flex kit is intended for the quantification of human IgE in solution. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Analysis of serum/plasma with this kit requires the use of ELISA diluent. The ELISA diluent blocks heterophilic antibodies from cross-linking the assay antibodies, thereby preventing false-positive read-outs. Heterophilic antibodies are commonly found in human serum/plasma. The ELISA diluent has been validated using serum/plasma samples from healthy human blood donors. It should be used for dilution of standard, samples, and detection antibody.

Recommendation

Product details

Product	ELISA Flex: Human IgE (HRP)
Application	ELISA
Analyte	IgE
Reactivity	Human
Specificity	This kit is based on a matched pair of mAbs specific for human IgE.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store antibodies and enzyme conjugate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C.
Shelf life	At least 18 months from date of receipt.

Performance

Standard range

0.3-30 ng/ml

Representative standard curve for human IgE ELISA Flex kit using TMB substrate.

Documents

Datasheet/Protocol SDS

Human IgE mAb guide

Selecting the appropriate mAbs is key to detecting both total and antigen-specific human IgA. This blog post explores Mabtech’s mAbs, tailored for ELISpot to detect human IgA antibody-secreting cells.

Application guide: ELISA

Antigen-specific IgE

Coating:
Antigen
Detection mAbs:
107/182/101, biotin

Total IgE

Coating:
107, unconjugated
Detection mAbs:
182, biotin

Application guide: ELISpot

Total IgE

Coating mAb:
107, unconjugated
Detection mAbs:
107/182/101, biotin

Get inspired!

Make sure to check out how researchers have used Mabtech's human IgE ELISA, ELISpot, and FluoroSpot kits in allergy research. You can find it all in our Publication database.

Tutorials

Tutorial, Published September 20, 2023

ELISA kit with HRP or ALP – what to choose?

You are interested in one of our ELISA Flex kits but wondering whether to choose the kit based on ALP or HRP? It’s a matter of taste, but we'd say HRP is mainly used for the colorimetric reaction. Find out why.

Tutorial, Published July 31, 2023

ELISA publications by sample type

Find out how researchers have used our ELISAs to analyze different types of samples.

Tutorial, Published November 28, 2022

How to detect immunoglobulins with ELISA

Detect and quantify immunoglobulins in solution, either antigen-specific or even total Ig.

Tutorial, Published November 28, 2022

Step-by-step guide to ELISA

Learn how to successfully run your next ELISA experiment with our step-by-step guide to the ELISA protocol.

Product highlights

Behind the scenes, Published March 27, 2023

We received an award

We are humbled to learn that we received an award from CiteAb. Based on citations for our ELISA kits, Mabtech has been highly commended in the 'ELISA Kit Supplier to Watch in 2023’ category.

Publications (18)

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Analyte information

IgE

Analyte description	The main physiological function of immunoglobulin E (IgE) is to protect against parasitic infestation. However, IgE is also associated with allergy, and allergen-specific IgE antibodies may trigger allergic reactions in the presence of the allergen. IgE is normally found in low amounts in serum/plasma but is significantly increased in allergic individuals, and the detection of allergen-specific IgE serves as the basis for many in vitro assays for allergy diagnosis.
Alternative names	Immunoglobulin E, IgE
Cell type	B cell
Gene ID	3497