Spot analysis reinvented
To make plate reading and spot analysis effortless and reliable, we developed Mabtech IRIS™ 2 from the ground up. Relying on our years of extensive knowledge in the ELISpot and FluoroSpot field, we knew first hand what the issues were with other readers, and launched our very own back in 2018.
Currently on our second generation, Mabtech IRIS 2 supports FluoroSpot, ELISpot, and FociSpot analysis.
Our readers offer a revolutionary approach to spot analysis. Simply select the assay, load the plate, and press read. Our patented RAWspot™ algorithm ensures highly accurate spot center detection and counting. The Mabtech Apex™ software provides a plug-and-play system that is incredibly user-friendly. With IRIS 2, you can enjoy effortless and accurate spot analysis.
Yes, reinvented!
Mabtech IRIS™ 2 is a next-generation 4-color FluoroSpot and ELISpot reader using RAWspot technology that ensures reliable multiplexing.
A new dimension of data
Every spot has a volume corresponding to the relative amount of secreted analyte
And a new era of plug-and-play
Simply load your 96-well plate and press “Read” – IRIS automatically handles calibration, focus, plate positioning, and light exposure, delivering true plug-and-play reading with distortion-free imaging.
Detect every spot
Our readers identify distinct spots in a linear fashion without hitting a plateau. Based on signal processing, the algorithm provides objective results for immediate analysis. Every spot, no matter how small, big, faint, or distinct, is detected.
Why Mabtech IRIS?
Mabtech IRIS 2 delivers plug-and-play ELISpot, FluoroSpot, and FociSpot analysis with minimal setup – just load the plate, select your assay, and press “read.”
IRIS uses the patented RAWspot™ algorithm and intuitive Apex™ software to ensure accurate spot center detection and reliable counting with reduced user bias. With support for up to four-color FluoroSpot and integration of FociSpot applications, IRIS 2 streamlines complex analyses while enabling deep data exploration and export for downstream tools.
Self-calibration, fixed focus, and readiness for automation make IRIS a fast, reproducible solution for both routine and high-throughput workflows.
How to analyze FluoroSpot on Mabtech IRIS
Discover the power of automation as a robotic arm flawlessly loads ELISpot plates into the Mabtech IRIS 2.
Reader specifications
Hardware
| XY table | Self-calibrating |
| Light source(s) | FluoroSpot: LED380, LED490, LED550, LED640 ELISpot: White LED ring light |
| Camera sensor | CMOS sensor with global shutter |
| Camera lens | Telecentric |
| Resolution (H x W) | 2048 x 2048 pixels |
| Plate types | 96-well ELISpot: MSIP, MAIPSWU FluoroSpot: IPFL FociSpot: Corning, Nunc |
| Dimensions (H x W x D) | 505 x 430 x 480 mm |
| Computer | Desktop PC included |
| Robotic automation | Ready |
Software
| Mabtech Apex™ | RAWspot™ technology included |
| Export formats | .raw, .jpg, .xlsx, .pzfx |
| Reading speed | ELISpot: ~1:30 min/plate FluoroSpot: ~5–15 min/plate FociSpot: <2 min/plate |
| Operating system | Windows |
| Compliance | CE, RoHS, REACH, WEEE, FCC, ICES, CFR21 part 11, EU Annex 11 |
Upgrade your existing reader!
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