Tell the story of every cell
FluoroSpot is the multiplex version of ELISpot. Just like in ELISpot, target proteins secreted by cells are captured by specific antibodies immediately after secretion and throughout the stimulation, making it an extremely sensitive sandwich assay. The difference to ELISpot lies in the mode of detection: where ELISpot relies on an enzymatic reaction, FluoroSpot utilizes fluorescence.
Confidence in every well
We know your samples are precious and your results must be trusted. That’s why we pair proven assay technology with direct access to experienced support scientists. If something doesn’t go as planned, we’ll work with you to get it right. Because our goal isn’t just an assay that runs, it’s an assay that delivers trustworthy results, every single time.
FluoroSpot you can trust
FluoroSpot from Mabtech means quality through and through. You’re getting more than a kit, you’re getting years of assay innovation, optimized protocols, and the tools you need to generate data you can trust to drive your research and therapies forward.
Decades of expertise: Our first FluoroSpot kits were launched in 2008, and ever since, we’ve been advancing the technology, with trusted antibodies and kits used in hundreds of peer-reviewed studies and clinical trials.
Exceptional sensitivity: Detects even the rarest antigen-specific T and B cells, ensuring no important response goes undetected.
Polyfunctional assessment: The multiplexing capacity of FluoroSpot allows for evaluating cells secreting more than one analyte, giving even more detailed immune insights.
Relative spot volume: The patented RAWspot™ algorithm allows for measurement of the relative amount of analyte of interest secreted, adding more nuanced insights in your immune profiling.
In-house antibody development: Proprietary monoclonal antibodies give unmatched specificity and affinity, batch after batch.
Integrated reading solutions: Mabtech IRIS™ 2 with the patented RAWspot™ algorithm provides the highest precision in spot detection, counting, and polyfunctional assessment available.
T cell applications
FluoroSpot is commonly used to investigate antigen-specific immune responses and to discriminate between subsets of activated T cells. This is applied in studies of infectious diseases, cancer, allergies, and autoimmune diseases. In vaccine research, IFN-γ/IL-2/TNF-α FluoroSpot is heavily used to define vaccine efficacy by measuring the capacity to elicit potent T cell responses. IFN-γ/IL-2/Granzyme B FluoroSpot is another great combination to assess both Th1 and cytotoxic T cells in the same well, saving on precious samples.
Immunotherapy
FluoroSpot is a powerful assay in CAR-T, TIL, and gene therapy development, giving clear insight into cytokine release such as IFN-γ, Granzyme B, and TNF-α from engineered T cells or virally transduced cells. These assays align with FDA recommendations for immunotherapy potency testing.
Vaccines
In vaccine development, FluoroSpot enables researchers to screen immune memory, identify vaccine target epitopes, evaluate novel adjuvants, and assess response durability by detecting and characterizing specific T cell subsets.
B cell applications
The B cell FluoroSpot assay is one of few assays that can measure immunoglobulins directly upon secretion. One can assess multiple total immunoglobulin secretion by antibody-secreting cells (ASCs) or memory B cells, as well as multiple antigen-specific B cell responses from both cell types, using the antigens of interest as a capture or detection reagent.
Evaluate cross-reactive B cells
B cell FluoroSpot can be used to answer several research questions in infection and vaccine research. One can assess multiple immunoglobulin isotypes, subclasses, and even antigens! By using reverse B cell FluoroSpot, multiple antigens can be used to detect single and cross-reactive B cells. This adds new, added layers to your B cell research and can help guide new vaccine development and assessment.
FluoroSpot kit formats
FluoroSpot kit
Build your own kit
Build your own FluoroSpot kit with thousands of combinations to choose from. Choose your analytes, and the appropriate plates and stimuli are included.
Total assay time:
- 3-day protocol
- 2-step detection
Kit size options:
- 1- or 10-plate kits
- Bulk options available upon request
FluoroSpot kit
Reproducible
Our most popular FluoroSpot kits with validated analyte combinations and everything you need for the assay including a positive control (for human and NHP kits).
Total assay time:
- 3-day protocol
- 2-step detection
Kit size options:
- 2- or 10-plate kits
- Bulk options available upon request
FluoroSpot kit
Antigen-specific
A complete FluoroSpot kit to answer your specific scientific question with validated analyte combinations, an included PepPool and positive control. Just add cells!
Total assay time:
- 3-day protocol
- 2-step detection
Kit size options:
- 1-plate kit
- Bulk options available upon request
Can't find a FluoroSpot Plus combination that suits your study? We offer custom coating services for most analyte combinations. Just contact your local account manager and we can discuss the project further!
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