ELISA
An ELISA is only as good as the antibodies that capture and detect the analyte of interest. Our meticulously validated antibodies ensure exceptional sensitivity and specificity.
Detect the real protein – not just the recombinant
We apply the exact same scrutiny when we choose the optimal antibody pair for our ELISA kits as we do with our ELISpot kits. Often, we have validated the same antibody pair for ELISpot, FluoroSpot, and ELISA in parallel. We offer ELISAs for selected cytokines, chemokines, immunoglobulins, and apolipoproteins for a range of species.
Award-winning ELISA kits
We are humbled that we received an award from CiteAb for our ELISAs twice. In both 2019 and 2023, Mabtech was highly commended in the "ELISA Kit Supplier to Watch" category, based on citations for our ELISA kits. A big thank you to CiteAb for spreading data-driven knowledge on research tools. Together we support researchers in making a difference.
Learn how a sandwich ELISA works
Detect native proteins
Antibody features such as affinity, avidity, and antigen interaction are essential for a successful ELISA. At Mabtech, we put considerable effort into characterizing these antibodies, and we always validate our kits for recognition of native proteins as this often differs from how the antibodies bind to recombinant proteins.
Confidence in every well
Your samples are precious, and your ELISA results must be trusted. That’s why we deliver rigorously validated ELISA assays backed by direct access to experienced support scientists. Because it’s not enough that an assay runs – it has to deliver accurate, reproducible results every time.
Prevent false-positive results
False-positive signals caused by rheumatoid factor and heterophilic antibodies like HAIA or HAMA are a common challenge in sandwich ELISA, particularly when working with clinical samples.
Our ELISA kits are designed to eliminate this interference, delivering accurate and reliable results even in demanding sample types. For the most difficult samples (e.g., autoimmune), we offer a specialized format, ELISA PathRF.
When your data are clean and trustworthy, you can focus on what matters most – generating results that stand up to peer review, like more than 1500 of our customers.
Applications
Broad sample range
Apolipoproteins
Immunoglobulin detection
T cell response
Measuring T cell responses is critical to understanding cellular immunity in infections, cancer, and autoimmunity. Interferon-gamma release assays (IGRA) offer a specific way to assess T cell activation by detecting IFN-γ secretion after antigen stimulation. Widely used in clinical and research settings, such as tuberculosis screening and vaccine studies, IGRAs can be adapted for diverse disease contexts. Looking for extra sensitivity? Explore our ELISpot kits!
ELISA kit formats
ELISA Flex
Adaptable
Flex kits are our most adaptable kit format, allowing you to customize them to your liking. Add your preferred substrate, plates, and buffers to complete this setup.
Total assay time:
- 2-day protocol
Kit size options:
- Reagents for 6 and 20 plates
- Bulk options available upon request
ELISA Pro
Validated
Use our ELISA Pro kits to minimize variability between different experiments. These kits are thoroughly validated and include every reagent necessary.
Total assay time:
- 1-day protocol
Kit size options:
- 1-, 2-, and 10-plate kits
- Bulk options available upon request
ELISA PathRF
RF-blocking
We recommend using our ELISA PathRF kits for clinical samples, particularly from autoimmune disease patients, as these kits are optimized to resist interference from rheumatoid factor (RF) and heterophilic antibodies.
Total assay time:
- 1-day protocol
Kit size options:
- 1-plate kits
- Bulk options available upon request
HRP or ALP?
Our ELISA Flex kits are almost always available in two versions: they rely on either ALP or HRP for the colorimetric reaction. Looking for help deciding between the two? Check out these tips.
Custom order?
If you’re interested in bulk orders, customizing kit components, requesting pre-coated plates, or purchasing reagents separately, please contact us. We’d be happy to discuss how we can accommodate your specific needs.
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