ELISA substrate: TMB for HRP

ELISA substrate: TMB for HRP

3652-F10

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ELISA/ELISpot conjugate: Streptavidin‑HRP
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ELISA/ELISpot conjugate: Streptavidin-HRP

Product specifications

Intended use

This ELISA substrate is intended for the development of ELISA assays using Streptavidin-HRP enzyme conjugates. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Description
This high sensitivity TMB substrate is suitable for ELISA assays with horseradish peroxidase (HRP). The substrate is supplied as a ready-to-use solution and produces a soluble blue product. Addition of an acid, e.g. sulfuric acid, stops the enzymatic reaction, which changes the color from blue to yellow. The absorbance of the stopped reaction is measured at 450 nm.

Recommendation

The substrate should preferably be used cold. Add the substrate after incubation with horseradish peroxidase and subsequent washing. Incubate for typically 5-15 minutes and then stop the enzymatic reaction by adding an acid, e.g. 0.18 M H2SO4. This will change the color from blue to yellow. Read the absorbance at 450 nm.

Product details

ProductELISA substrate: TMB for HRP
ApplicationELISA
Contents

Ready-to-use TMB substrate for ELISA 

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store at 4-8 °C upon receipt.

Shelf lifeAt least 12 months from date of receipt.
Datasheet/ProtocolSDS
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