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ELISA substrate: TMB for HRP
ELISA substrate: TMB for HRP
3652-F10
In stock
Delivery 4-9 business days
Shipping $0
Complementary products
Complementary products
Intended use
This ELISA substrate is intended for the development of ELISA assays using Streptavidin-HRP enzyme conjugates. For research use only. Not for use in diagnostic procedures.
Serum/Plasma samples
Description
This high sensitivity TMB substrate is suitable for ELISA assays with horseradish peroxidase (HRP). The substrate is supplied as a ready-to-use solution and produces a soluble blue product. Addition of an acid, e.g. sulfuric acid, stops the enzymatic reaction, which changes the color from blue to yellow. The absorbance of the stopped reaction is measured at 450 nm.
Recommendation
The substrate should preferably be used cold. Add the substrate after incubation with horseradish peroxidase and subsequent washing. Incubate for typically 5-15 minutes and then stop the enzymatic reaction by adding an acid, e.g. 0.18 M H2SO4. This will change the color from blue to yellow. Read the absorbance at 450 nm.
Product details
| Product | ELISA substrate: TMB for HRP |
| Application | ELISA |
| Contents | Ready-to-use TMB substrate for ELISA |
Shipping and Storage
| Shipping | Shipped at ambient temperature. |
| Storage | Store at 4-8 °C upon receipt. |
| Shelf life | At least 12 months from date of receipt. |