ELISA Flex: Human IFN-γ (HRP)

ELISA Flex: Human IFN‑γ (HRP)

3420-1H-6

Publications: 243

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An ELISA designed to detect the native protein – in our most adaptable kit format to match your unique setup. Combine it with your preferred substrate and buffer.

Components

Capture mAb	1-D1K
Detection mAb	7-B6-1, biotin
Enzyme conjugate	Streptavidin-HRP
Standard	Recombinant human IFN-γ ELISA standard
Buffer/Solution	Standard reconstitution buffer

In stock

Delivery 4-9 business days

Shipping $0

Complementary products

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ELISA diluent

Product specifications

Intended use

This ELISA Flex kit is intended for the quantification of human IFN-γ in solution. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Analysis of serum/plasma with this kit requires the use of ELISA diluent. The ELISA diluent blocks heterophilic antibodies from cross-linking the assay antibodies, thereby preventing false-positive read-outs. Heterophilic antibodies are commonly found in human serum/plasma. The ELISA diluent has been validated using serum/plasma samples from healthy human blood donors. It should be used for dilution of standard, samples, and detection antibody.

Recommendation

Product details

Product	ELISA Flex: Human IFN-γ (HRP)
Application	ELISA
Analyte	IFN-γ
Reactivity	Human
Specificity	This kit is based on a matched pair of mAbs specific for human IFN-γ.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store at 4-8 °C upon receipt.
Shelf life	At least 18 months from date of receipt.

Performance

Standard range

4-400 pg/ml

Representative standard curve for human IFN-γ ELISA Flex kit using pNPP substrate.

Documents

Datasheet/Protocol
SDS

Product highlights

Behind the scenes, Published March 27, 2023

We received an award

We are humbled to learn that we received an award from CiteAb. Based on citations for our ELISA kits, Mabtech has been highly commended in the 'ELISA Kit Supplier to Watch in 2023’ category.

Tutorials

Tutorial, Published July 31, 2023

ELISA publications by sample type

Find out how researchers have used our ELISAs to analyze different types of samples.

Tutorial, Published November 28, 2022

Step-by-step guide to ELISA

Learn how to successfully run your next ELISA experiment with our step-by-step guide to the ELISA protocol.

Publications (243)

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Analyte information

IFN-γ

Analyte description	Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.
Alternative names	Interferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg, IFG, IFI, IMD69
Cell type	T cell, Tc, Th1, NK cell
Gene ID	3458