ELISpot conjugate: Streptavidin-ALP

ELISpot conjugate: Streptavidin‑ALP

3310-10-1000

Publications: 64
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Content

Streptavidin-ALP enzyme conjugate

In stock

Delivery 4-9 business days

Shipping $0

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ELISpot substrate: BCIP/NBT-plus for ALP


Product specifications

Intended use

This Streptavidin-ALP enzyme conjugate is intended for ELISpot asays. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Description
Streptavidin-ALP enables detection of biotinylated antibodies and biotinylated antigens. This Streptavidin-ALP conjugate is optimized for ELISpot. The conjugate has high residual enzyme activity and biotin affinity. For ELISA assays, we recommend using . 

Recommendation

Dilute 1:1000 as a general guideline.

Product details

ProductELISpot conjugate: Streptavidin-ALP
ApplicationELISpot
Conjugation

The preparation has been thoroughly depleted of free ligand and free enzyme. The conjugate has high residual enzyme activity and biotin affinity.

Supplied in

0.1 M Tris buffer with 1% BSA and 0.002% Kathon CG.

Contents

Streptavidin-ALP enzyme conjugate

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store at 4-8 °C upon receipt.

Shelf lifeAt least 18 months from date of receipt.
Datasheet/Protocol
SDS

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot
We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.

Highlighted research, Published November 8, 2022

Modified ELISpot to detect viral proteins
Detection of secreted antigens by HIV infected cells and quantification of CD4 T cells with adapted ELISpot viral protein spot assay (VIP-Spot).
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