This ready-to-use BCIP/NBT-plus substrate is intended for the development of ELISpot assays using Streptavidin-ALP enzyme conjugates. For research use only. Not for use in diagnostic procedures.
Serum/Plasma samples
Recommendation
Adjust to room temperature before use. Filter the substrate solution through a 0.45 µm filter and add to ELISpot plates after incubation with alkaline phosphatase and subsequent washing. Develop until spots emerge. Stop the colour development by washing extensively in tap water.