ELISpot Flex: Human IgG2 (ALP)

ELISpot Flex: Human IgG2 (ALP)

3852-2A

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Our most adaptable ELISpot format, made to fit into your unique plan. Combine it with a substrate, plate, and/or antigen of choice.

Components

Capture mAbMT145
Detection mAbMTG211E, biotin
Enzyme conjugateStreptavidin-ALP
StimuliR848
Recombinant human IL-2

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Delivery 4-9 business days

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ELISpot plates, white


Product specifications

Intended use

This ELISpot Flex kit is intended for the enumeration of cells secreting human IgG2 using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

With this kit, detection of antigen-specific IgG2 secreting B cells can be performed using coated antigen. If detection with biotinylated antigen is preferred, the plate should instead be coated with the subclass specific antibody.

For analysis of memory B cells, we recommend pre-stimulation with R848 and recombinant human IL-2 (both included).

Recommendation

Product details

ProductELISpot Flex: Human IgG2 (ALP)
ApplicationELISpot
AnalyteIgG2
ReactivityHuman
Specificity

This kit is based on a matched pair of mAbs enabling specific detection of human IgG2.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies and enzyme conjugate at 4-8 °C upon receipt. R848 and IL-2 should be stored at -20 °C.

Shelf lifeAt least 18 months from date of receipt.
PBMC were pre-incubated in the presence or absence of R848 and recombinant IL-2. The cells were then washed, added into ELISpot wells (100,000 cells/well) and analyzed for the secretion of total IgG2 (overnight incubation).
Datasheet/Protocol
SDS

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Isolation, freezing, and thawing of PBMCs
This is a protocol on how to best isolate, freeze, and thaw PBMCs.
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IgG2

Analyte description

Immunoglobulin G (IgG) is the most abundant Ig isotype in serum, making up approximately 80% of all serum Immunoglobulins. In humans, there are four subclasses of IgG, with the highest serum concentrations of IgG1 followed by IgG2, IgG3, and IgG4. IgG2 is a poor activator of Fc receptors. 

Alternative namesImmunoglobulin G2, IgG2
Cell typeB cell
Gene ID3501