ELISA Pro: Human apoE
ELISA Pro: Human apoE
Components
| Plate | Pre-coated ELISA strip plate (mAb E276) |
| Detection mAb | E887, biotin |
| Enzyme conjugate | Streptavidin-HRP |
| Standard | Recombinant human apoE ELISA standard |
| Substrate | ELISA substrate: TMB for HRP |
| Buffers/Solutions | Apo ELISA buffer concentate |
| Standard reconstitution buffer | |
| Streptavidin-HRP diluent | |
| Wash buffer concentrate | |
| Stop solution | |
| Also provided | Adhesive plate covers |
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Performance
| Standard range | 0.02-15 ng/ml |
| Sensitivity | 0.01 ng/ml The lowest concentration that can be detected, but not necessarily quantified with precision and accuracy. This was determined by adding 2 standard deviations to the mean OD of background wells. |
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Non-human primate cross-reactivity guide
The systems reactive with NHPs are either based on cross-reactive human kits or specifically developed monkey kits (NHP).
Cross-reactivity verification tests have been performed in ELISpot and/or ELISA by Mabtech and/or by others. Evaluations of assays with less solid evidence of cross-reactivity are shown as symbols within parentheses.
| ApoA1 | |||||||||
| ApoB | |||||||||
| ApoE (NHP) | |||||||||
| ApoE | |||||||||
| ApoH | |||||||||
| CCL2 (MCP-1) | |||||||||
| CCL4 (MIP-1β) | ( ) | ( ) | ( ) | ( ) | ( ) | ||||
| CCL22 (MDC) | ( ) | ( ) | ( ) | ( ) | ( ) | ||||
| CD25 | |||||||||
| GM-CSF | ( ) | ( ) | |||||||
| Granzyme A | |||||||||
| Granzyme B (NHP) | |||||||||
| Granzyme B | |||||||||
| IFN-α2 | |||||||||
| IFN-α pan | ( ) | ||||||||
| IFN-γ (NHP) | |||||||||
| IFN-γ | ( ) | ||||||||
| IgA (NHP) | |||||||||
| IgA | |||||||||
| IgG | |||||||||
| IgM | |||||||||
| INS | |||||||||
| IL-1α | |||||||||
| IL-1β | |||||||||
| IL-2 (NHP) | |||||||||
| IL-3 | |||||||||
| IL-4 | ( ) | ( ) | ( ) | ( ) | |||||
| IL-5 | ( ) | ( ) | ( ) | ||||||
| IL-6 | ( ) | ( ) | ( ) | ||||||
| IL-8 (NHP) | |||||||||
| IL-8 (ELISA) | |||||||||
| IL-8 (ELISpot) | |||||||||
| IL-10 (NHP) | |||||||||
| IL-10 | |||||||||
| IL-12/-23 (p40) | |||||||||
| IL-12 (p70) | |||||||||
| IL-13 (NHP) | ( ) | ( ) | ( ) | ||||||
| IL-13 | |||||||||
| IL-17A (NHP) | ( ) | ( ) | |||||||
| IL-17A | |||||||||
| IL-17F | |||||||||
| IL-17A/F | |||||||||
| IL-21 | |||||||||
| IL-22 | |||||||||
| IL-23 | |||||||||
| IL-27 | |||||||||
| IL-31 | |||||||||
| IP-10 | |||||||||
| Perforin | ( ) | ||||||||
| TGF-β1 (latent) | |||||||||
| TNF-α (NHP) | ( ) | ( ) | ( ) | ( ) | ( ) | ||||
| TNF-α | |||||||||
| CD3, mAb CD3-1 | |||||||||
| CD3, mAb CD3-2 | |||||||||
| CD28, mAb CD28-A | |||||||||
| IFN-γ mAb 1-D1K | ( ) | ( ) | ( ) | ( ) | ( ) | ( ) | |||
| IL-2 mAb MT8G10 | ( ) | ( ) | ( ) | ( ) | ( ) | ( ) | ( ) | ||
| IL-4 mAb IL4-3 | ( ) | ||||||||
| IL-17A mAb MT504 | |||||||||
| Perforin mAb Pf-344 | |||||||||
| TNF-α mAb MT15B15 | |||||||||
| IgG1 mAb MTG1218 | |||||||||
| IgG1 mAb MT1939 | |||||||||
| IgG2 mAb H6200 | |||||||||
| IgG2 mAb MTG211E | |||||||||
| IgG3 mAb MTG34 | |||||||||
| IgG4 mAb MTG42 | |||||||||
| IFN-γ mAb MT111W | ( ) | ( ) | ( ) | ( ) | ( ) | ( ) | ( ) | ( ) | ( ) |
| TNF-α mAb MT15B15 | ( ) | ( ) | ( ) | ( ) | ( ) | ( ) | ( ) |
Symbol keys:
Verified by Mabtech and/or by others. Tests performed in ELISpot and/or ELISA.
- good
- poor
- no
Based on reports from others or by analysis using recombinant proteins.
- ( ) good
- ( ) poor
- ( ) no
Specifically developed monkey kits are marked with (NHP).
Blank boxesRepresent cross-reactivities not evaluated.
* Comprises several species. Cross-reactivity may have to be verified on a species basis.
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Analyte information
apoE
| Analyte description | Apolipoprotein E (apoE) is a 34 kD glycoprotein involved in lipid transport, lipoprotein metabolism, and cellular signaling. It is present in plasma lipoprotein particles such as chylomicrons, VLDL, and HDL. ApoE is also present in large amounts at sites of neurological damage and appears to be involved in the recycling of apoptotic remnants and amyloidal aggregates. ApoE is mainly produced in the liver and by macrophages, but also by other cell types such as smooth muscle cells or neuronal cells. In humans, apoE exists in three isoforms, apoE2, apoE3, and apoE4. Of these, apoE4 is associated with Alzheimer’s disease. A normal concentration of apoE in human plasma is approximately 30-40 µg/ml. The concentration of ApoE in inbred mouse plasma can vary between 15-150 µg/ml. |
| Alternative names | Apolipoprotein E, apoE, apolipoproteinE, apo E, AD2, APO-E, ApoE4, LDLCQ5, LPG |
| Cell type | T cell, Treg, Tfh |
| Gene ID | 348 |