FluoroSpot Plus: Mouse IFN-γ/IL-4

FluoroSpot Plus: Mouse IFN‑γ/IL‑4

FSP-4146-2

Publications: 4
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Ensure reproducibility when diving into a multiplex analysis.

Components

PlatePre-coated FluoroSpot plate (mAbs AN18 and 11B11)
Detection mAbsAnti-IFN-γ mAb (R4-6A2), BAM
Anti-IL-4 mAb (BVD6-24G2), biotin
Fluorophore conjugatesAnti-BAM mAb, 490
SA-550
Buffer/SolutionFluoroSpot enhancer
Co-stimulusAnti-CD28 mAb

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This FluoroSpot Plus kit is intended for the enumeration of cells secreting mouse IFN-γ and IL-4 using the FluoroSpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductFluoroSpot Plus: Mouse IFN-γ/IL-4
ApplicationFluoroSpot
AnalyteIFN-γ, IL-4
ReactivityMouse
SpecificityThis kit is based on matched pairs of mAbs specific for mouse IFN-γ and IL-4.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore antibodies, fluorophore conjugates, and FluoroSpot enhancer at 4-8 °C upon receipt. Plates may be kept at room temperature.
Shelf lifeAt least 12 months from date of receipt.
Mouse splenocytes (250,000 cells/well) were incubated overnight in the absence or presence of anti-CD3/anti-CD28 antibodies and the number of cells secreting IFN-γ and/or IL-4 was analyzed by FluoroSpot. Double-secreting cells were determined as spots having the same center point in an image overlay of LED490 (IFN-γ) and LED550 (IL-4) images. Spot analysis was made with Mabtech IRIS.

Tutorial, Published November 24, 2022

Cell stimulation in ELISpot and FluoroSpot
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IFN-γ

Analyte description

Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.

Alternative namesInterferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg, If2f, Ifg
Cell typeT cell, Tc, Th1, NK cell
Gene ID15978

IL-4

Analyte description

Interleukin 4 (IL-4) is produced primarily by Th2 cells, mast cells, eosinophils, and basophils. IL-4 was first identified to costimulate B cell growth; the cytokine is essential for B cell secretion of IgE and augments the production of IgG1 in vivo. In addition to regulating B cell growth and immunoglobulin secretion, IL-4 also affects T cells. In vitro, IL-4 promotes T cell growth and can induce cytolytic T cell activity. IL-4 also induces the differentiation of naive CD4 T cells into Th2 cells, which are characterized by their capacity to secrete the cytokines IL-4, IL-5, and IL-10 upon activation, while simultaneously inhibiting the generation of Th1 cells.

Alternative namesInterleukin 4, IL-4, IL4, BCGF-1, BSF-1, Il-4
Cell typeT cell, Th2, Treg
Gene ID16189