Components
Plate | Pre-coated FluoroSpot plate (mAbs AN18 and 11B11) |
Detection mAbs | Anti-IFN-γ mAb (R4-6A2), BAM |
Anti-IL-4 mAb (BVD6-24G2), biotin | |
Fluorophore conjugates | Anti-BAM mAb, 490 |
SA-550 | |
Buffer/Solution | FluoroSpot enhancer |
Co-stimulus | Anti-CD28 mAb |
In stock
Delivery 4-9 business days
Shipping $0
Performance
Documents
Tutorials
Publications (4)
Analyte information
IFN-γ
Analyte description | Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens. |
Alternative names | Interferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg, If2f, Ifg |
Cell type | T cell, Tc, Th1, NK cell |
Gene ID | 15978 |
IL-4
Analyte description | Interleukin 4 (IL-4) is produced primarily by Th2 cells, mast cells, eosinophils, and basophils. IL-4 was first identified to costimulate B cell growth; the cytokine is essential for B cell secretion of IgE and augments the production of IgG1 in vivo. In addition to regulating B cell growth and immunoglobulin secretion, IL-4 also affects T cells. In vitro, IL-4 promotes T cell growth and can induce cytolytic T cell activity. IL-4 also induces the differentiation of naive CD4 T cells into Th2 cells, which are characterized by their capacity to secrete the cytokines IL-4, IL-5, and IL-10 upon activation, while simultaneously inhibiting the generation of Th1 cells. |
Alternative names | Interleukin 4, IL-4, IL4, BCGF-1, BSF-1, Il-4 |
Cell type | T cell, Th2, Treg |
Gene ID | 16189 |