ELISA Pro: Human apoD

ELISA Pro: Human apoD

3714-1HP-1

Publications: 5
Documents
Questions?
Thoroughly validated and include every buffer necessary, for a reliable and reproducible assay.

Components

PlatePre-coated ELISA strip plate (mAb D544)
Detection mAbD263, biotin
Enzyme conjugateStreptavidin-HRP
StandardNative human apoD ELISA standard
SubstrateELISA substrate: TMB for HRP
Buffers/SolutionsApo ELISA buffer concentate
Standard reconstitution buffer
Streptavidin-HRP diluent
Wash buffer concentrate
Stop solution
Also providedAdhesive plate covers

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This ELISA Pro kit is intended for the quantification of human apoD in serum, plasma, and cell culture supernatants. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

The Apo ELISA buffer included in this kit blocks heterophilic antibodies from cross-linking the assay antibodies, thereby preventing false-positive read-outs. Heterophilic antibodies are commonly found in human serum/plasma and can also be present in other species. The Apo ELISA buffer has been validated using serum/plasma samples from healthy human blood donors.

Apolipoprotein dilution
Apolipoproteins are present at high levels in serum and plasma. For ELISA quantification, such samples must therefore be diluted 5,000-200,000 times depending on the apolipoprotein analyzed.

Recommendation

Product details

ProductELISA Pro: Human apoD
ApplicationELISA
AnalyteapoD
ReactivityHuman
Specificity

This kit is based on a matched pair of mAbs specific for human apoD.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store detection mAb, Streptavidin-HRP, buffers/solutions, and substrate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C. Plates may be kept at room temperature.

Shelf lifeAt least 12 months from date of receipt.
Standard range1-32 ng/ml
Sensitivity0.44 ng/ml

The lowest concentration that can be detected, but not necessarily quantified with precision and accuracy. This was determined by adding 5 standard deviations to the mean OD of background wells.

Mean standard curve from repeated runs of human apoD ELISA Pro kit. The complete curve indicates the recommended standard dilution while the standard range is shown in bold.
Datasheet/Protocol
SDS

Tutorial, Published July 31, 2023

ELISA publications by sample type
Find out how researchers have used our ELISAs to analyze different types of samples.

Tutorial, Published November 28, 2022

Step-by-step guide to ELISA
Learn how to successfully run your next ELISA experiment with our step-by-step guide to the ELISA protocol.

Tutorial, Published November 14, 2022

Apolipoprotein dilution
Recommendations for Mabtech's apolipoprotein ELISAs

Behind the scenes, Published March 27, 2023

We received an award
We are humbled to learn that we received an award from CiteAb. Based on citations for our ELISA kits, Mabtech has been highly commended in the 'ELISA Kit Supplier to Watch in 2023’ category. 
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apoD

Analyte description

Apolipoprotein D (apoD) is a lipocalin that binds lipids and steroid hormones. The apoD gene is expressed in many tissues, e.g. brain, testis, adrenal gland, kidney, placenta, spleen, lung, and pancreas. ApoD likely functions as a transporter of lipids or lipid hormones, and is involved in the interaction between HDL and LDL. ApoD gene polymorphisms are associated with obesity, hyperinsulinemia, and type 2 diabetes. Normal concentration of apoD in human plasma is approximately 0.05-0.15 g/L.

Alternative namesApolipoprotein D, apoD, apolipoproteinD, apo D
Gene ID347