ELISA Pro: Mouse apoA1

3750-1HP-1

Publications: 9

Documents

Questions?

Thoroughly validated and include every buffer necessary, for a reliable and reproducible assay.

Components

Plate	Pre-coated ELISA strip plate (mAb mHDL93)
Detection mAb	mHDL36, biotin
Enzyme conjugate	Streptavidin-HRP
Standard	Native mouse apoA1 ELISA standard
Substrate	ELISA substrate: TMB for HRP
Buffers/Solutions	Sample diluent
	Standard reconstitution buffer
	Dilution buffer
	Wash buffer concentrate
	Stop solution
Also provided	Adhesive plate covers

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This ELISA Pro kit is intended for the quantification of mouse apoA1 in serum, plasma, and cell culture supernatants. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Apolipoprotein dilution
Apolipoproteins are present at high levels in serum and plasma. For ELISA quantification, such samples must therefore be diluted 5,000-200,000 times depending on the apolipoprotein analyzed.

Recommendation

Product details

Product	ELISA Pro: Mouse apoA1
Application	ELISA
Analyte	apoA1
Reactivity	Mouse
Specificity	This kit is based on a matched pair of mAbs specific for mouse apoA1.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store detection mAb, Streptavidin-HRP, buffers/solutions, and substrate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C. Plates may be kept at room temperature.
Shelf life	At least 12 months from date of receipt.

Performance

Standard range	0.16-10 ng/ml
Sensitivity	0.03 ng/ml The lowest concentration that can be detected, but not necessarily quantified with precision and accuracy. This was determined by adding 5 standard deviations to the mean OD of background wells.

Mean standard curve from repeated runs of mouse apolipoprotein A1 (apoA1) ELISA Pro kit.

Documents

Datasheet/Protocol
SDS

Tutorials

Tutorial, Published July 31, 2023

ELISA publications by sample type

Find out how researchers have used our ELISAs to analyze different types of samples.

Tutorial, Published November 28, 2022

Step-by-step guide to ELISA

Learn how to successfully run your next ELISA experiment with our step-by-step guide to the ELISA protocol.

Tutorial, Published November 14, 2022

Apolipoprotein dilution

Recommendations for Mabtech's apolipoprotein ELISAs

Product highlights

Behind the scenes, Published March 27, 2023

We received an award

We are humbled to learn that we received an award from CiteAb. Based on citations for our ELISA kits, Mabtech has been highly commended in the 'ELISA Kit Supplier to Watch in 2023’ category.

Highlighted research, Published September 27, 2018

Immunological effects of hyperlipidemia

First citations for Mabtech ELISA mouse apoE and apoA1 investigating immunological effects of hyperlipidemia.

Publications (9)

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Analyte information

apoA1

Analyte description	Apolipoprotein A1 (apoA1) is the main protein component of high-density lipoprotein particles (HDL). ApoA1 (28 kD) is mainly produced in the liver and exported into circulation where it acquires lipids and becomes HDL. A high level of apoA1 is considered protective against atherosclerosis and cardiovascular disease. The normal concentration of apoA1 in human plasma is approximately 0.8-2.2 g/L.
Alternative names	Apolipoprotein A1, apoA1, apolipoprotein-A1, apo A1, apoA-I, Alp-1, Apoa-1, Brp-14, Ltw-1, Lvtw-1, Sep-1, Sep-2, Sep2, apo-AI
Gene ID	11806