ELISpot Path: TB (ESAT-6+CFP-10+EspC) Human IFN-γ

ELISpot Path: TB (ESAT‑6+CFP‑10+EspC) Human IFN‑γ

3420-2AST-P4-1

Publications: 0
Documents
Questions?
A straightforward setup for functional analysis of a specific scientific question.

Components

PlatePre-coated ELISpot plate, strip (mAb 1-D1K)
Detection mAb7-B6-1, ALP
SubstrateBCIP/NBT-plus
Positive controlAnti-CD3 mAb (CD3-2)
Peptide poolsTB (ESAT-6), scanning
TB (CFP-10), scanning
TB (EspC), scanning

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This ELISpot Path kit is intended for the enumeration of cells secreting human IFN-γ in response to TB peptides using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Peptide pool composition
The scanning pools consist of synthetic peptides of 15-mers overlapping with 11 amino acids, covering the complete proteins. The ESAT-6 scanning pool is derived from the early secretory antigenic target protein and contains 21 peptides. The CFP-10 scanning pool is derived from the culture filtrate protein and contains 23 peptides. Finally, the EspC scanning pool is derived from the ESX-1 secretion-associated protein and contains 23 peptides.

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Product details

ProductELISpot Path: TB (ESAT-6+CFP-10+EspC) Human IFN-γ
ApplicationELISpot
AnalyteIFN-γ
ReactivityHuman
Specificity

This kit is based on a matched pair of mAbs specific for human IFN-γ.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies, enzyme conjugate, and substrate at 4-8 °C. Peptide pools should be stored at -20 °C or below. Plates may be kept at room temperature.

Shelf lifeAt least 18 months from date of receipt.
PBMC (250,000 cells/well) from an individual with latent tuberculosis were incubated overnight in the absence or presence of the ESAT-6, CFP-10, and EspC scanning pools (Mabtech) and the number of cells secreting IFN-γ was analyzed by ELISpot.
Datasheet/Protocol
SDS

Highlighted research, Published October 28, 2024

Immunoassays for infectious disease research
Explore the role of immunoassays like ELISpot and FluoroSpot in infectious disease research. Discover how Mabtech's immunoassays enable researchers to study immune responses at the cellular level for diseases like TB, SARS-CoV-2, Malaria, HIV, and more.

Highlighted research, Published October 28, 2024

ELISpot vs ELISA: Superior sensitivity in TB diagnostics
ELISpot is preferred over ELISA in TB diagnostics due to its higher sensitivity, enabling early detection of TB-specific T cells through single-cell analysis of IFN-γ secretion. ELISpot offers a more accurate immune response assessment, crucial for diagnosing TB in humans and animals.

Highlighted research, Published December 21, 2023

White paper: Tuberculosis diagnosis with spots
Early and precise diagnosis is crucial for effective TB management, and ELISpot is increasingly being used for this purpose. This white paper examines the advantages of ELISpot in TB diagnosis and suggests future antigens and biomarkers that can enhance diagnostic accuracy.

Behind the scenes, Published November 17, 2023

Webinar: Novel insights into TB vaccines and diagnostics
Recording now available!

Featured product, Published September 2, 2021

Our TB peptide pools go Path
Our PepPools for tuberculosis were combined into ELISpot and FluoroSpot Path kits.

Tutorial, Published November 24, 2022

Cell stimulation in ELISpot and FluoroSpot
In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.

Tutorial, Published November 7, 2022

7 common ELISpot errors and how to avoid them
ELISpot troubleshooting. We discuss 7 of the most common issues we’ve encountered in ELISpot, what mistakes causes them and how to avoid and correct them.

Tutorial, Published October 14, 2022

What is a positive ELISpot response?
DFR is a non-parametric statistical test for determining positive responses in ELISpot and FluoroSpot.

Tutorial, Published September 2, 2022

Isolation, freezing, and thawing of PBMCs
This is a protocol on how to best isolate, freeze, and thaw PBMCs.
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IFN-γ

Analyte description

Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.

Alternative namesInterferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg, IFG, IFI, IMD69
Cell typeT cell, Tc, Th1, NK cell
Gene ID3458
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FluoroSpot Path: TB (ESAT-6+CFP-10+EspC) Human IFN-γ/IL-2