Peptide pool with 100 peptides, 25 ug of each peptide. Supplied in one vial (lyophilized).
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Product specifications
Intended use
This peptide pool is intended for studying immune responses against SARS-CoV-2 (spike protein) using immunoassays such as ELISpot and FluoroSpot. It induces cytokine secretion from antigen-specific human T cells. For research use only. Not for use in diagnostic procedures.
Serum/Plasma samples
Description
The SARS-CoV-2 (S) defined peptide pool induces secretion of e.g., IFN-γ, IL-2, and granzyme B from human T cells. It contains 100 peptides from the human SARS-CoV-2 virus. The peptides are derived from the spike (S) protein. The mean purity of the synthetic peptides is 90%.
The peptide pool has been validated using human PBMC from COVID-19 convalescent individuals and COVID-19 vaccinated individuals.
Published HLA types covered by the SARS-CoV-2 S defined peptide pool are listed in the datasheet.
Recommendation
Product details
Product
PepPool: SARS-CoV-2 (S), human
Application
T cell stimulation, ELISpot, FluoroSpot, Flow cytometry
Reactivity
Human
Supplied in
Lyophilized
Contents
Peptide pool with 100 peptides, 25 ug of each peptide. Supplied in one vial (lyophilized).
Shipping and Storage
Shipping
Shipped at ambient temperature.
Storage
Store at -20 °C or below upon receipt.
Performance
Human PBMC (250,000 cells/well) from one COVID-19 vaccinated individual (mRNA vaccine with spike sequence), one COVID-19 convalescent individual, and from one individual collected before the SARS-CoV-2 pandemic were incubated overnight in the absence or presence of the SARS-CoV-2 S defined peptide pool and anti-CD28 mAb. The number of cells secreting IFN-γ was analyzed by ELISpot using Mabtech IRIS.
Human PBMC (250,000 cells/well) from one COVID-19 vaccinated individual (mRNA vaccine with spike sequence), one COVID-19 convalescent individual, and from one individual collected before the SARS-CoV-2 pandemic were incubated overnight in the absence or presence of the SARS-CoV-2 S defined peptide pool and anti-CD28 mAb. The number of cells secreting IFN-γ, IL-2 and both analytes was analyzed by FluoroSpot using Mabtech IRIS.