PepPool: SARS-CoV-2 (S), human

PepPool: SARS‑CoV‑2 (S), human

3630-1

Publications: 6
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Validated for our assays.

Content

Peptide pool with 100 peptides, 25 ug of each peptide. Supplied in one vial (lyophilized).

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Product specifications

Intended use

This peptide pool is intended for studying immune responses against SARS-CoV-2 (spike protein) using immunoassays such as ELISpot and FluoroSpot. It induces cytokine secretion from antigen-specific human T cells. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Description
The SARS-CoV-2 (S) defined peptide pool induces secretion of e.g., IFN-γ, IL-2, and granzyme B from human T cells. It contains 100 peptides from the human SARS-CoV-2 virus. The peptides are derived from the spike (S) protein. The mean purity of the synthetic peptides is 90%.

The peptide pool has been validated using human PBMC from COVID-19 convalescent individuals and COVID-19 vaccinated individuals.

Published HLA types covered by the SARS-CoV-2 S defined peptide pool are listed in the datasheet.

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Product details

ProductPepPool: SARS-CoV-2 (S), human
ApplicationT cell stimulation, ELISpot, FluoroSpot, Flow cytometry
ReactivityHuman
Supplied in

Lyophilized

Contents

Peptide pool with 100 peptides, 25 ug of each peptide. Supplied in one vial (lyophilized).

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store at -20 °C or below upon receipt. 

Human PBMC (250,000 cells/well) from one COVID-19 vaccinated individual (mRNA vaccine with spike sequence), one COVID-19 convalescent individual, and from one individual collected before the SARS-CoV-2 pandemic were incubated overnight in the absence or presence of the SARS-CoV-2 S defined peptide pool and anti-CD28 mAb. The number of cells secreting IFN-γ was analyzed by ELISpot using Mabtech IRIS.

Highlighted research, Published October 28, 2024

Immunoassays for infectious disease research
Explore the role of immunoassays like ELISpot and FluoroSpot in infectious disease research. Discover how Mabtech's immunoassays enable researchers to study immune responses at the cellular level for diseases like TB, SARS-CoV-2, Malaria, HIV, and more.
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