Intended use
This peptide pool is intended for studying immune responses against the spike protein of SARS-CoV-2 (variant Omicron B.1.1.529, linage BA.1) using immunoassays such as ELISpot and FluoroSpot. It induces cytokine secretion from antigen-specific human T cells. For research use only. Not for use in diagnostic procedures.
Description
The SARS-CoV-2 Omicron BA.1 (S1) scanning pool induces secretion of e.g., IFN-γ and IL-2 from human T cells. It contains 168 peptides from the human SARS-CoV-2 virus variant Omicron (B.1.1.529), lineage BA.1. The peptides are 15-mers overlapping with 11 amino acids, covering the S1 domain of the spike protein. The peptide pool contains mutations: A67V, Δ69-70, T95I, G142D, Δ143-145, Δ211, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, and P681H of Omicron BA.1 spike S1.
The peptide pool has been validated using human PBMC from COVID-19 convalescent individuals and vaccinated individuals.
The pool is supplied in two vials: Pool 1: SARS-CoV-2 (Omicron BA.1) S1 peptides 1-84 (84 peptides) and pool 2: SARS-CoV-2 (Omicron BA.1) S1 peptides 85-168 (84 peptides). The mean purity of the synthetic peptides is 80%.
Peptides included in the peptide pool are listed in the datasheet.