PepPool: EBV (CD4 and CD8), human

PepPool: EBV (CD4 and CD8), human

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Peptide pool with 100 peptides, 25 ug of each peptide (lyophilized).

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Product specifications

Intended use

This peptide pool is intended for studying immune responses against EBV using immunoassays such as ELISpot, FluoroSpot, and flow cytometry. It induces cytokine secretion from antigen-specific human CD4 and CD8 T cells. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Description
The EBV peptide pool induces secretion of e.g., IFN-γ and IL-2 from human T cells. It contains 100 peptides derived from 22 of the latent and lytic EBV proteins. Of the peptides, 66 are MHC class I-restricted, and 34 are MHC class II-restricted. The mean purity of the synthetic peptides is 90%.

HLA types covered by the peptide pool:
HLA-A*01, HLA-A*02, HLA-A*03, HLA-A*11, HLA-A*24, HLA-A*25, HLA-A*26, HLA-A*68, HLA-B*07, HLA-B*08, HLA-B*15, HLA-B*18, HLA-B*23, HLA-B*25, HLA-B*26, HLA-B*27, HLA-B*35, HLA-B*38, HLA-B*40, HLA-B*44, HLA-B*55, HLA-B*57, HLA-B*58, HLA-DRB1*01, HLA-DRB1*03, HLA-DRB1*04, HLA-DRB1*07, HLA-DRB1*08, HLA-DRB1*11, HLA-DRB1*13, HLA-DRB1*15, HLA-DRB1*16, HLA-DRB3*01, HLA-DRB3*02, HLA-DRB4*01, HLA-DRB5*01, HLA-DPB1*104, HLA-DPB1*04, HLA-DPB4*01, HLA-DQB1*06, HLA-DQB1*07

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Product details

ProductPepPool: EBV (CD4 and CD8), human
ApplicationT cell stimulation, ELISpot, FluoroSpot, Flow cytometry
ReactivityHuman
Supplied in

Lyophilized

Contents

Peptide pool with 100 peptides, 25 ug of each peptide (lyophilized).

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store at -20 °C or below upon receipt. 

Human PBMC (250,000 cells/well) were incubated overnight in the absence or presence of the EBV defined peptide pool (Mabtech) and the number of cells secreting IFN-γ was analyzed by ELISpot.

Highlighted research, Published October 28, 2024

Immunoassays for infectious disease research
Explore the role of immunoassays like ELISpot and FluoroSpot in infectious disease research. Discover how Mabtech's immunoassays enable researchers to study immune responses at the cellular level for diseases like TB, SARS-CoV-2, Malaria, HIV, and more.
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