3619-1
Content
Peptide pool with 42 peptides, 25 ug of each peptide. Supplied in one vial (lyophilized).
In stock
Delivery 4-9 business days
Shipping $0
Product specifications
Intended use
This peptide pool is intended for studying immune responses against CMV in immunoassays such as ELISpot, FluoroSpot, and flow cytometry. It induces cytokine secretion from antigen-specific human CD4 and CD8 T cells. For research use only. Not for use in diagnostic procedures.
Serum/Plasma samples
Description
The CMV peptide pool induces secretion of e.g., IFN-γ, IL-2, IL-5, and IL-13 from human CD4 and CD8 T cells. It contains 42 peptides from cytomegalovirus. The antigens represented in the peptide pool are pp50, pp65, IE1, IE2, and envelope glycoprotein B. 28 of the peptides are MHC class I-restricted and 14 are MHC class II-restricted. The synthetic peptides are >95% pure.
HLA types covered by the CMV pool:
HLA class I: HLA-A1, HLA-A2, HLA-A3, HLA-A11, HLA-A23, HLA-A24, HLA-A26, HLA-A30, HLA-B7, HLA-B8, HLA-B18, HLA-B27, HLA-B35, HLA-B40/60, HLA-B41, HLA-B44, HLA-B57/58, HLA-C7. HLA class II: HLA-DRB1*01, 03, 04, 05, 07, 08, 11, 15, 20, 24, 53, HLA-DPw2, HLA-DQB, HLA-DP3*14, 20
Recommendation
Product details
Product | PepPool: CMV (CD4 and CD8), human |
Application | T cell stimulation, ELISpot, FluoroSpot, Flow cytometry |
Reactivity | Human |
Supplied in | Lyophilized |
Contents | Peptide pool with 42 peptides, 25 ug of each peptide. Supplied in one vial (lyophilized). |
Shipping and Storage
Shipping | Shipped at ambient temperature. |
Storage | Store at -20 °C or below upon receipt. |
Performance
Human PBMC stimulated with CMV peptide pool (2 ug/ml) and anti-CD28 antibody (0.1 ug/ml) for 16 hours (both available from Mabtech) in the presence of Brefeldin A. In donor 1, CD4+ cells start expressing IFN-γ following CMV peptide pool stimulation whereas donor 2 responds by production of IFN-γ by CD8+ cells. Cells were stained with CD4-PE and CD8-FITC prior to fixation and permeabilization using 4% paraformaldehyde and saponin. IFN-γ was detected using 1D1K-PF647P (Mabtech). The dot plots derive from gated events of typical lymphocyte characteristics in forward and side light scatter. Flow cytometry was performed on a BD FACSVerse system.
Documents
Publications (9)
You may also like