PepPool: AdV5 (hexon), human

PepPool: AdV5 (hexon), human

3634-1

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Content

Peptide pool with 94 peptides, 25 ug of each peptide. Supplied in one vial (lyophilized).

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Delivery 4-9 business days

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Product specifications

Intended use

This peptide pool is intended for studying immune responses against adenovirus serotype 5 (AdV5) using immunoassays such as ELISpot and FluoroSpot. It induces cytokine secretion from antigen-specific human T cells. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Description
This AdV5 (hexon) peptide pool induces secretion of e.g., IFN-γ and IL-2 from human T cells. It contains 94 peptides derived from the human adenovirus 5 (hAdV5) hexon protein. It consists of both defined and scanning peptides. The scanning peptides are 15-mers overlapping with 11 amino acids. Together, these peptides cover immunodominant regions of the hAdV5 hexon protein that share sequence homology to human adenovirus 26 and chimpanzee adenovirus Y25. The mean purity of the synthetic peptides is 80%.

The peptide pool has been validated in ELISpot and FluoroSpot using human PBMC.

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Product details

ProductPepPool: AdV5 (hexon), human
ApplicationT cell stimulation, ELISpot, FluoroSpot, Flow cytometry
ReactivityHuman
Supplied in

Lyophilized

Contents

Peptide pool with 94 peptides, 25 ug of each peptide. Supplied in one vial (lyophilized).

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store at -20 °C or below upon receipt. 

Human PBMC (250,000 cells/well) were incubated overnight in the absence or presence of the Adenovirus 5 hexon peptide (Mabtech) and the number of cells secreting IFN-γ was analyzed by ELISpot.
Datasheet/ProtocolSDS
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FluoroSpot Path: AdV5 (hexon) Human IFN-γ/IL-2