ELISpot Path: TBEV (C​+​E) Human IFN‑γ

Human PBMC (250,000 cells/well) were incubated for 20 h in the absence or presence of PepPool: TBEV (C+E), scanning and the number of cells secreting IFN-γ was analyzed by ELISpot. Analysis was done with Mabtech IRIS. 

ELISpot Path: TBEV (C​+​E) Human IFN‑γ

3420-4AST-P8-1

$670

Publications: 0
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Bulk or custom format
A straightforward setup for functional analysis of a specific scientific question.

Components

PlatePre-coated ELISpot plate, strip (mAb 1-D1K)
Detection mAb7-B6-1, biotin
Enzyme conjugateStreptavidin-ALP enzyme conjugate for ELISpot
SubstrateBCIP/NBT-plus substrate for ELISpot
Positive controlAnti-CD3 mAb (CD3-2)
Peptide poolTBEV (C+E), scanning

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This ELISpot Path kit is intended for the enumeration of cells secreting human IFN-γ in response to tick-borne encephalitis virus (TBEV) peptides. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Peptide pool composition
PepPool: TBEV (C+E), scanning contains 75 synthetic peptides derived from TBEV. 26 peptides are from the capsid (C) protein and 49 peptides from the envelope (E) protein. The peptides are 15-mers overlapping with 11 amino acids.

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Product details

ProductELISpot Path: TBEV (C+E) Human IFN-γ
ApplicationELISpot
AnalyteIFN-γ
ReactivityHuman
Specificity

This kit is based on a matched pair of mAbs specific for human IFN-γ.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies, enzyme conjugate, and substrate at 4-8 °C. The peptide pool should be stored at -20 °C or below. Plates may be kept at room temperature.

Shelf lifeAt least 18 months from date of receipt.
Human PBMC (250,000 cells/well) were incubated for 20 h in the absence or presence of PepPool: TBEV (C+E), scanning and the number of cells secreting IFN-γ was analyzed by ELISpot. Analysis was done with Mabtech IRIS. 

Human PBMC (250,000 cells/well) were incubated for 20 h in the absence or presence of PepPool: TBEV (C+E), scanning and the number of cells secreting IFN-γ was analyzed by ELISpot. Analysis was done with Mabtech IRIS. 

Datasheet/ProtocolSDS

Highlighted research
Published: October 28, 2024 | 18 minute read

Immunoassays for infectious disease research

Explore the role of immunoassays like ELISpot and FluoroSpot in infectious disease research. Discover how Mabtech's immunoassays enable researchers to study immune responses at the cellular level for diseases like TB, SARS-CoV-2, Malaria, HIV, and more.

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Published: October 28, 2024 | 1 minute read

Pre-existing TBEV immunity skews B cell response to Yellow Fever vaccine

This study investigates B cell responses to yellow fever vaccination in individuals with prior TBEV immunity. ELISpot identifies skewed antibody production, highlighting cross-reactive IgG and antibody-dependent enhancement (ADE) linked to flavivirus fusion loop epitopes.

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IFN-γ

Analyte description

Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.

Alternative namesInterferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg, IFG, IFI, IMD69
Cell typeT cell, Tc, Th1, NK cell
Gene ID3458