B cells

ELISpot and FluoroSpot are some of the most sensitive assays for detecting and quantifying antigen-specific B cells. These methods allow for the assessment of both memory B cells and active antibody-secreting cells, evaluating their antigen-specificity as well as immunoglobulin isotype or subclass. Complement these assays with ELISA to quantify levels of total or antigen-specific immunoglobulin secretion.

Tutorial, Published November 21, 2022

B cell profiling with ELISpot and FluoroSpot
ELISpot and FluoroSpot are considered the gold standard method for finding rare, antigen-specific B cells.

Behind the scenes, Published June 11, 2024

Webinar recording: B cell profiling with ELISpot
Learn about B cell profiling using ELISpot and FluoroSpot assays.

Tutorial, Published January 12, 2023

Step-by-step Memory B cell stimulation
Get your memory B cells ready for ELISpot/FluoroSpot with our StimPacks (included in all B cell ELISpot/FluoroSpot kits)

Tutorial, Published November 28, 2022

How to detect immunoglobulins with ELISA
Detect and quantify immunoglobulins in solution, either antigen-specific or even total Ig.

Highlighted research, Published May 30, 2020

B cell FluoroSpot for sensitive ASC detection
Mabtech IRIS and IgG/IgA/IgM FluoroSpot were used to study antibody-secreting plasma cells (ASCs) specific for the nucleocapsid (N) protein. FluoroSpot was shown to be more sensitive than e.g., ELISA.

Featured product, Published June 8, 2021

Tetanus toxoid, biotin – designed for B cell profiling
This tagged antigen could complement your next reverse B cell ELISpot & FluoroSpot.

Highlighted research, Published October 15, 2019

Detection of tumor infiltrating B cells
Tumor infiltrating B cells are able to actively secrete immunoglobulins as shown by performing an IgA, IgG and IgM FluoroSpot. The FluoroSpot technology enabled investigation of the functionality of cells directly isolated from the tumor.